Status: Planned Paper
Section: Stem Cells
Type of Paper: Full Research Paper
Title: “The tumorigenic risk of adipose tissue derived stem cells in vitro”
Authors: Yiwei Qian, MD 1,  Jianhua Gao, MD, PhD 1,  Feng Lu, MD, PhD 1,2, Xudong Zheng, MD 1
Affiliations:
1. Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China
2. Division of Plastic Surgery, Brigham and Women’s Hospital, Harvard Medical School, Boston, USA
Abstract: To investigate the safety of adipose-derived stem cells(ASCs) for transplantation, compared the biological characteristics of adipose-derived stem cells and lipoma-derived mesenchymal stem cells (LMSCs) in vitro. ASCs and LMSCs were isolation and the appearance of cultured cells was observed. The cell viability was evaluated with MTS chromatometry and cell growth curve was generated. Flow cytometry was performed for cell cycle analysis and the expression of the cell surface marker profiles. QRT-PCR was used to detect the expression of tumor-specific gene (the high-mobility group AT-hook 2, HMGA2), and immunocytochemistry was used to detect the expression of telomerase. Apparent differences was observed in histologic sections of the tissues; ASCs are similar to LMSCs in cell shape; ASCs shown a significant lower proliferation capacity than LMSCs by MTS chromatometry; The expressions of CD29, CD44, CD105 in ASCs was similar to LMSCs while the level of CD133 was a significant lower in ASCs(5.35%) than in LMSCs(26.87%). ASCs shown a lower proliferation capacity than LMSCs by cell cycle analysis; The expression of HMGA2 shown a lower level in ASCs(RQ=1) than in LMSCs(RQ=1.79) by qRT-PCR; And in ASCs and LMSCs, the integrated optical intensity(IOD) values of hTERT expression are 1379.597±498.617 and 3328.108±902.856, size(area) are 132390.27±35568.945 and 238000.53±49264.289, density(mean) are 0.009±0.003 and 0.014±0.003. It presented significant differences between ASCs and LMSCs in the biological characteristics in vitro experiment, and no evidence indicate the malignant transformation of ASCs, however, to confirm the conclusion, it needs further long-term series of experiments.
Keywords: Adipose-derived mesenchymal stem cells (ASCs) ;  Lipoma-derived mesenchymal stem cells (LMSCs) ;  Tumorigenicity